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更新日期：2022年6月29日
姓 名 文继开 性 别 男
出生年月 1977年7月 籍贯 当阳市
民 族 汉族 政治面貌 中共党员
最后学历 博士研究生 最后学位
技术职称 教授 导师类别 博、硕导
行政职务 Email jkwen@scau.edu.cn
工作单位 生物化学及分子生物学系 邮政编码 510642
通讯地址 广州天河区五山路483号华南农业大学
单位电话 020-85201331
个人主页
个人简介
主要从事真菌毒素的毒理机制和代谢转化研究，聚焦于动物体内的细胞应激、稳态维持和基因表达调控研究。已在EMBO J，Nature Structural Molecular Biology，Cell Death and Differentiation等国际知名期刊发表40余篇SCI论文。主持国家自然科学基金面上项目、十三五重点研发子课题、广东省教育厅重大基础研究项目等。
工作经历
2014.01- 华南农业大学生命科学学院 教授
2010.06-2013.12 伯明翰大学生物系 Research Fellow
2009.11-2010.05 伯明翰大学生物系 Research Assistant
2002.08-2005.10 中国科学院武汉病毒研究所 助理研究员
教育经历
博士 博士研究生 理学 细胞生物学 伯明翰大学 全日制 2005-10 2010-01
硕士 硕士研究生 理学 生物化学与分子生物学 中国科学院武汉病毒研究所 全日制 1999-08 2002-07
获奖、荣誉称号
大北农科技奖创新奖（2022） 排名3； 广东省教学成果特等奖（2022） 排名4
社会、学会及学术兼职
广东省农业生物蛋白质功能与调控重点实验室 副主任；广东省生化与分子生物学会 常务理事；广东省生物物理学会 常务理事
研究领域
1.兽医毒理学：霉菌毒素的毒理机制与代谢转化

2. 生物化学与分子生物学：外界胁迫下动物细胞应激与稳态维持机制
科研项目
在研课题
（1）国家“十三五”重点研发计划，2017YFC1600305、畜禽体内微生物毒素的协同侵染规律与危害形成机制、2017/12-2022/11、在研、主持。
（2）广东省教育厅重大基础研究项目，2018KZDXM01、单端孢酶烯族毒素的脱毒代谢机制、2019/04-2022/03、在研、主持。
（3）国家自然科学基金NSFC-广东联合基金重点项目，U1901207、真菌毒素（呕吐毒素）致猪中毒的发病机制与防控基础研究、2020/01-2023/12、在研、参与。

已完成课题
（1）广东省自然科学基金自由申请项目，2014A030313458、外源化合物引发畜禽细胞P450核糖核酸多样性的研究、 2015/01-2017/12、已结题、主持。
（2）国家自然科学基金面上项目，34171234、出芽酵母中RNA剪接依赖的无义介导的mRNA衰变的机理研究、2015/01-2018/12、已结题、主持。
（3）广州市科技计划项目，201607010177、外源毒素引发畜禽体内细胞色素P450蛋白多样性的研究、2016/04-2019/03、20万元、已结题、主持。
发表论文
代表性论著
1. Ruqin Lin, Danyang Li, Yangyang Xu, Mengyao Wei, Qingmei Chen, Yiqun Deng*, and Jikai Wen*. Chronic cereulide exposure causes intestinal inflammation and gut microbiota dysbiosis in mice. Environmental Pollution 2021 288 (2021) 117814. https://doi.org/10.1016/j.envpol.2021.117814
2. Fengru Deng, Yunsheng Chen, Xiaoyu Zhou, Huiying Xiao, Tianyu Sun, Yiqun Deng*, and Jikai Wen*. New insights into the virulence traits and antibiotic resistance of enterococci isolated from diverse probiotic products. Microorganisms 2021, 9, 726. https://doi.org/10.3390/microorganisms9040726
3. Qingmei Chen#, Dong Men#, Tianyu Sun#, Yan Zhang, Qianqian Yuan, Taidou Hu, Zhangsheng Hu, Jun Wu, Yiqun Deng, Xian-En Zhang*, and Jikai Wen*. Supreme Catalytic Properties of Enzyme Nanoparticles Based on Ferritin Self-Assembly. ACS Applied Bio Materials. 2020 Oct; 3(10) 7158–7167. doi.org/10.1021/acsabm.0c00961
4. Fengru Deng#, Yunsheng Chen#, Tianyu Sun, Yuting Wu, Yiting Su, Changyue Liu, Junyu Zhou, Yiqun Deng*, and Jikai Wen*. Antimicrobial resistance, virulence characteristics and genotypes of Bacillus spp. from probiotic products of diverse origins. Food Research International. 2021 Jan; 139, 109949. doi.org/10.1016/j.foodres.2020.109949.
5. Han Qiao, Tianqing Jiang, Peiqiang Mu, Xiaoxuan Chen, Xianhui Wen, Zhangsheng Hu, Shulin Tang, Jikai Wen*, and Yiqun Deng*. Cell fate determined by the activation balance between PKR and SPHK1. Cell Death & Differentiation. 2020 Aug; doi.org/10.1038/s41418-020-00608-8.
6. Yurong Zhao, Shulin Tang, Ruqin Lin, Ting Zheng, Danyang Li, Xiaoxuan Chen, Jiahui Zhu, Jikai Wen*, and Yiqun Deng*. Deoxynivalenol Exposure Suppresses Adipogenesis by Inhibiting the Expression of Peroxisome Proliferator-Activated Receptor Gamma 2 (PPARγ2) in 3T3-L1 Cells. International Journal of Molecular Science. 2020 Aug; 21(17), 6300. doi.org/10.3390/ijms21176300.  
7. Ruqin Lin, Yu Sun, Peiqiang Mu, Ting Zheng, Haibin Mu, Fengru Deng, Yiqun Deng*, and Jikai Wen*. Lactobacillus rhamnosus GG supplementation modulates the gut microbiota to promote butyrate production, protecting against deoxynivalenol exposure in nude mice. Biochemical Pharmacology. 2020 May; 175, 113868. doi.org/10.1016/j.bcp.2020.113868.
8. Shulin Tang#, Yurong Zhao#, Xirong He, Jiahui Zhu, Shuang Chen, Jikai Wen*, and Yiqun Deng*. Identification of NOVA family proteins as novel beta-catenin RNA-binding proteins that promote epithelial-mesenchymal transition. RNA Biology. 2020 Jun;17(6):881-891. doi: 10.1080/15476286.2020.1734372.
9. Zhangsheng Hu#, Yu Sun#, Jiongjie Chen, Yurong Zhao, Han Qiao, Ruohong Chen, Xianhui Wen, Yiqun Deng*, and Jikai Wen*, Deoxynivalenol globally affects the selection of 3' splice sites in human cells by suppressing the splicing factors, U2AF1 and SF1. RNA Biology. 2020 Apr; 17(4):584-595. doi: 10.1080/15476286.2020.1719750. Epub 2020 Feb 6.
10. Yu Sun#, Jikai Wen#, Ruohong Chen, and Yiqun Deng*. Variable protein homeostasis in housekeeping and non-housekeeping pathways under mycotoxins stress. Scientific Reports. 2019 May 24; 9(1), 7819.
11. Wenchu Ye, Ruohong Chen, Xiaoxuan Chen, Boyan Huang, Ruqin Lin, Xuan Xie, Jiongjie Chen, Jun Jiang, Yiqun Deng*, and Jikai Wen*. AhR regulates the expression of human cytochrome P450 1A1 (CYP1A1) by recruiting Sp1. FEBS Journal. 2019 Jun 14; 286 (21), 4215-4231.
12. Wenchu Ye, Ruqin Lin, Xiaoxuan Chen, Jiongjie Chen, Ruohong Chen, Xuan Xie, Yiqun Deng*, and Jikai Wen*. T-2 toxin upregulates the expression of human cytochrome P450 1A1 (CYP1A1) by enhancing NRF1 and Sp1 interaction. Toxicology Letters.2019 Oct 15; 315, 77-86.
13. Ruqing Lin, Yu Sun, Wenchu Ye, Ting Zheng, Jikai Wen* and Yiqun Deng*. T-2 toxin inhibits the production of mucin via activating the IRE1/XBP1 pathway. Toxicology. 2019 Aug 1; 424:152230. (IF：3.547，JCRQ1区)
14. Shulin Tang, Shuang Chen, Boyan Huang, Jun Jiang, Jikai Wen*, and Yiqun Deng*. Deoxynivalenol induces inhibition of cell proliferation via the Wnt/beta-catenin signaling pathway. Biochemical Pharmacology. 2019 May 7; 166, 12-22.  
15. Xuan Xie#, Jun Jiang, Wenchu Ye, Ruohong Chen, Yiqun Deng* and Jikai Wen*. Sp1, Instead of AhR, Regulates the Basal Transcription of Porcine CYP1A1 at the Proximal Promoter. Frontiers in Pharmacology. 2018 Aug 17; 9:927. doi: 10.3389/fphar.2018.00927. (IF：3.831，医学2区)
16. Ruohong Chen, Jun Jiang, Zhangsheng Hu, Wenchu Ye, Qianqian Yuan, Mengyuan Li, Jikai Wen*, and Yiqun Deng*. Coordinated Transcriptional Regulation of Cytochrome P450 3As by Nuclear Transcription Factor Y and Specificity Protein 1. Molecular Pharmacology 2019 May; 95:507–518. (IF：3.982，医学2区)
17. Jikai Wen #, Peiqiang Mu #, and Yiqun Deng. Mycotoxins: cytotoxicity and biotransformation in animal cells, Toxicol Res. 2016 Jan 7; 5, 377-387.
18. Xin Liu#, Jikai Wen#, Ruohong Chen, Tingting Zhang, Jun Jiang, and Yiqun Deng*. T-2 toxin induces the expression of porcine CYP3A22 via the upregulation of the transcription factor, NF-Y. Biochimica et Biophysica Acta-General Subjects. 2016 Oct;1860(10): 2191-2201.  (IF: 5.083, 生物2区)
19. Han Liu, Min Luo, and Jikai Wen*. mRNA stability in the nucleus. Journal of Zhejiang University-Science B. 2014 May;15, 444-454.
20. Khalid Al-Jubran #, Jikai Wen #, Akilu Abdullahi#, Subhendu Roy Chaudhury, Min Li, Preethi Ramanathan, Annunziata Matina, Sandip De, Kim Piechocki, Kushal Nivriti Rugjee, and Saverio Brogna. Visualization of the joining of ribosomal subunits reveals the presence of 80S ribosomes in the nucleus. RNA. 2013 Dec; 19, 1669-1683. (IF: 4.605, 生物2区)
21. Jikai Wen#, and Saverio Brogna. Splicing-dependent NMD does not require the EJC in Schizosaccharomyces pombe. EMBO Journal. 2010 May 5;29, 1537-1551. (IF: 9.7919, 生物1区)
22. Saverio Brogna, and Jikai Wen. Nonsense-mediated mRNA decay (NMD) mechanisms. Nature Structural & Molecular Biology. 2009 Feb; 16, 107-113. (IF: 13.338, 生物1区)
23. Jikai Wen#, and Saverio Brogna. Nonsense-mediated mRNA decay. Biochemical Society Transactions. 2008 Jun;36, 514-516.
24. Jikai Wen#, Xian-en Zhang, Zhe Cheng, Hong Liu, Yafeng Zhou, Zhiping Zhang, Jihong Yang, and Jiaoyu Deng. A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1. Biosensors and Bioelectronics 2004 Feb 15;19, 685-92. (IF: 7.780, 工程技术1区)
25. Shihua Wang, Jikai Wen, Yafeng Zhou, Zhiping Zhang, Ruifu Yang, Jibin Zhang, Jia Chen, and Xian-en Zhang. Identification and characterization of Bacillus anthracis by multiplex PCR on DNA chip. Biosensors and Bioelectronics  2004 Nov 1;20, 807-13. (IF: 7.780, 工程技术1区)

其它发表论著
1. Xiaojuan Gao, Peiqiang Mu, Xunhua Zhu, Xiaoxuan Chen, Shulin Tang, Yuting Wu, Xiang Miao, Xiaohan Wang, Jikai Wen, and Yiqun Deng*. Dual Function of a Novel Bacterium, Slackia sp. D-G6: Detoxifying Deoxynivalenol and Producing the Natural Estrogen Analogue, Equol. Toxins (Basel). 2020 Jan 26;12(2). pii: E85. doi: 10.3390/toxins12020085.
2. Qian Liu, Jikai Wen, Jiahui Zhu, Tingting Zhang, Yiqun Deng*, and Jun Jiang*. Aromatic hydrocarbon receptor regulates chicken cytochrome P450 1A5 transcription: A novel insight into T-2 toxin-induced gene expression and cytotoxicity in LMH cells. Biochemical Pharmacology. 2019 Oct; 168:319-329. doi: 10.1016/j.bcp.2019.07.023. Epub 2019 Jul 25.
3. Jun Wu, Sisi Zhu, Yunbo Wu, Tianqing Jiang, Lingling Wang, Jun Jiang, Jikai Wen, and Yiqun Deng*. Multiple CH/π Interactions Maintain the Binding of Aflatoxin B₁ in the Active Cavity of Human Cytochrome P450 1A2. Toxins (Basel). 2019 Mar 12;11(3). pii: E158. doi: 10.3390/toxins11030158.
4. Haoran Jiang, Jun Wu, Feiyong Zhang, Jikai Wen, Jun Jiang*, Yiqun Deng*. The critical role of porcine cytochrome P450 3A46 in the bioactivation of aflatoxin B1. Biochemical Pharmacology. 2018 Aug 22; 156: 177-185. doi: 10.1016/j.bcp.2018.08.030.
5. Xiaoming Li, Peiqiang Mu, Han Qiao, Jikai Wen, Yiqun Deng*. JNK-AKT-NF-κB controls P-glycoprotein expression to attenuate the cytotoxicity of deoxynivalenol in mammalian cells. Biochemical Pharmacology. 2018 Aug 16; 156: 120-134. doi: 10.1016/j.bcp.2018.08.020.
6. Jun Jiang, Shulin Tang, Jianhong Xia, Jikai Wen, Shuang Chen, Xiaodong Shu, Michael S. Y. Huen, Yiqun Deng*. C9orf140, a novel Axin1-interacting protein, mediates the negative feedback loop of Wnt/β-catenin signaling. Oncogene. 2018 May; 37(22): 2992-3005. doi: 10.1038/s41388-018-0166-7.
7. Xiaoming Li, Peiqiang Mu, Jikai Wen, Yiqun Deng*. Carrier-mediated and energy dependent uptake and efflux of deoxynivalenol in mammalian cells. Scientific Reports. 2017 Jul; 7: 5889. doi:10.1038/s41598-017-06199-8.
8. Linfeng Dong, Qingmei Chen, Xin Liu, Jikai Wen, Jun Jiang*, Yiqun Deng*. Role of Specificity Protein 1, Hepatocyte Nuclear Factor 1α, and Pregnane X Receptor in the Basal and Rifampicin-Induced Transcriptional Regulation of Porcine Cytochrome P450 3A46. Drug Metabolism and Disposition. 2015 Oct; 43(10): 1458-1467.
9. Jun Wu, Ruohong Chen, Caihui Zhang, Kangbai Li, Weiyin Xu, Lijuan Wang, Qingmei Chen, Peiqiang Mu, Jum Jiang, Jikai Wen, and Yiqun Deng*. Bioactivation and Regioselectivity of Pig Cytochrome P450 3A29 towards Aflatoxin B1. Toxins. 2016 Sep 12; 8(9): 267. doi: 10.3390/toxins8090267.
10. Kushal Nivriti Rugjee, Subhendu Roy Chaudhury, Khalid Al-Jubran, Preethi Ramanathan, Tina Matina, Jikai Wen, and Saverio Brogna. Fluorescent protein tagging confirms the presence of ribosomal proteins at Drosophila polytene chromosomes. PeerJ. 2013 Feb 12;1:e15; DOI 10.7717/peerj.15
11. Zhongdao Li, Jikai Wen, Yaning Lin, Shihua Wang, Peng Xue, Zhiping Zhang, Yafeng Zhou, Xiao Wang, Li Sui, Lijun Bi, and Xian-en Zhang. A Sir2-Like Protein Participates in Mycobacterial NHEJ. PLoS one 2011 May 26;6(5): e20045. doi: 10.1371/journal.pone.0020045.
12. Nicklas Bonander, Richard Aj Darby, Ljuban Grgic, Nagamani Bora, Jikai Wen, Saverio Brogna, David R Poyner, Michael Aa O'Neill, and Roslyn M Bill. Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield. Microb Cell Fact. 2009 Jan 29;8, 10. doi: 10.1186/1475-2859-8-10.
13. Feng Li, Zhiping Zhang, Jun Peng, Zongqiang Cui, Daiwen Pang, Ke Li, Hongping Wei, Yafeng Zhou, Jikai Wen, and Xian-en Zhang. Imaging viral behavior in Mammalian cells with self-assembled capsid-quantum-dot hybrid particles. Small 2009 Mar;5, 718-26.
14. Na Li, Zongqiang Cui, Jikai Wen, Zhiping Zhang, Hongping Wei, Yafeng Zhou, and Xian-en Zhang. Live cell imaging of protein interactions in poliovirus RNA replication complex using fluorescence resonance energy transfer (FRET). Biochem Biophys Res Commun. 2008 Apr 11;368, 489-94.
15. Saverio Brogna, Preethi Ramanathan, and Jikai Wen. UPF1 P-body localization. Biochemical Society Transactions. 2008 Aug;3636, 698-700.
16. Jibin Zhang, Xian-en Zhang, Yafeng Zhou, Lijun Bi, Zhiping Zhang, Shihua Wang, Yuanyuan Chen, Yongchao Guo, Jikai Wen, and Zhiniu Yu. Construction and Characterization of an Anti-Prion scFv Fusion Protein Pair for Detection of Prion Protein on Antibody Chip. Analytical Letters. 2007 Jan 01;40:5, 855-873.
17. Yongchao Guo, Yafeng Zhou, Xian-en Zhang, Zhiping, Zhang, Yanmei Qiao, Lijun Bi, Jikai Wen, Mifang Liang, and Jibin Zhang. Phage display mediated immuno-PCR. Nucleic Acids Res. 2006 May 8;34, e62.
18. Fan You, Yafeng Zhou, Xian-en Zhang, Zhi Huang, Lijun Bi, Zhiping Zhang, Jikai Wen, Yuanyuan Chen, Guibin Jiang, and Minghui Zheng. Cell-free bioassay for measurement of dioxins based on fluorescence enhancement of fluorescein isothiocyanate-labeled DNA probe. Anal Chem. 2006 Oct 15;78, 7138-44.
19. Zongqiang Cui, Zhiping Zhang, Xian-en Zhang, Jikai Wen, Yafeng Zhou, and Weihong Xie. Visualizing the dynamic behavior of poliovirus plus-strand RNA in living host cells. Nucleic Acids Res. 2005 Jun 7;33, 3245-52.
20. 文继开，张香玲，周亚凤，王建春，张治平，张先恩 (2003). 采用基因芯片检出一例丙型肝炎3型3b亚型的报告。病毒学杂志 19, 274-7.
科研创新
1. 文继开、孙天钰、邓诣群、吴骏、陈庆梅、母培强、邓凤如，玉米赤霉烯酮内酯水解酶RmZHD在降解大环内酯类抗生素中的应用，2021.05.04，中国，ZL201911327678.X
2. 文继开、陈庆梅、张艳、邓诣群、吴骏、蒋珺、胡泰斗、胡章盛，一种基于人铁蛋白的纳米酶及其制备方法，2022.04.08，中国，ZL201810470199.2
3. 文继开、陈韵声、邓凤如、邓诣群，一株特异性抑制MRSA且安全无毒的贝莱斯芽孢杆菌PH6菌株及其应用，2022.04.26，中国，ZL202011306360.6
4. 邓诣群、高小娟、母培强、陈庆梅、文继开，一株高效降解呕吐毒素的伊格尔兹氏菌Eggerthella sp.D II-9，2017.07.11，中国，ZL 201610785205.4
5. 邓诣群、母培强、高小娟、陈庆梅、文继开，伊格尔兹氏菌Eggerthella sp. DII -9在降解呕吐毒素中的应用，2018.03.06，中国，ZL201610785036.4
6. 邓诣群、高小娟、母培强、文继开、祝勋花、吴玉婷，一株史雷克氏菌及其在降解呕吐毒素中的应用，2021.05.25，中国，ZL201910493701.6.
7.  张先恩，邓教宇，黄旭，张治平，邵文海，文继开，陆红兵，刘强，一种基因芯片上的酶标信号检测方法，专利号：ZL 02 1 15833.9。
教学活动
本科课程： 微生物学（双语、全英）；生物化学；生命科学导论；生物产业发展概述。研究生课程：现代分子生物学；生命科学方法与技术。